Fig. 3.

Histological changes induced by renal ischemia/reperfusion are prevented by pirfenidone. a-c) Representative light microphotographs of kidney slides stained with PAS from sham, I/R, and IR + PFN groups (× 100 magnification). d) Quantification of damaged tubules, e) number of casts formed, f) number of detached tubules. The sham group is represented by white bars; the I/R group, by black bars; and the IR + PFN group, by gray bars. Six rats per group were studied. Data are shown as the means ± SD. * = p < 0.05 vs. sham and I/R + PFN groups and & = p < 0.05 vs. sham group. The significance of the differences between groups was assessed by ANOVA using the Bonferroni correction for multiple comparisons