Table 3. Statistically significant KEGG pathways enriched for genes having a fixed amino acid difference between A. cervicornis and A. palmata. The third column gives the number of genes in the pathway with one or more fixed difference(s), and the third reports what fraction they represent of all genes in the pathway. For instance, 67 of the genes are annotated as belonging to the ABC transporter pathway, and 12/67 = 0.18. Statistical significance determined using a two-tailed Fisher’s exact test. Notice that these p-values are not corrected for multiple tests. A randomization analysis indicates that adf02010 = ABC transporters is significant at a FDR of 0.0046. The number of predicted false positives are provided for each KEGG pathway.

Pathway	p-value	False Positives	# Genes	Fraction
adf02010 = ABC transporters	0.0015	< 1	12	0.18
adf00790 = Folate biosynthesis	0.019	1.13	5	0.21
adf03420 = Nucleotide excision repair	0.031	1.73	7	0.15
adf04933 = AGE-RAGE signaling pathway in diabetic complications	0.023	1.13	9	0.14
adf04310 = Wnt signaling pathway	0.037	2.42	12	0.12