Summary of small RNAs in An. gambiae mosquito tissues. Female mosquitoes were divided into differing tissue groups, fat body-abdominal walls (FB-Ab, purple), midgut (MG, green), ovary (OV, red), and remaining tissues comprised of mosquito head and thorax (R, black). For size distributions of small RNAs across mosquito tissue groups, (A-D) histograms of read length over read number show the diverse small RNA read distributions for each mosquito tissue group. For each tissue group, size-selected small RNAs of 20-24 nts highlighted in gray were put into four classifications (E-H): (1) All Reads, designated with black circles, represented total reads, (2) Annotated Reads, designated with black triangles, represented annotated miRNAs, (3) Multi-mapping Reads, designated with white squares, represented non-unique sequence reads, and (4) Unannotated Reads, designated with white diamonds, represented undetermined small RNAs. All values represent three biological replicates, and values are graphed as average +/− SEM.