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. 2019 Mar 21;9(5):1647–1654. doi: 10.1534/g3.119.400150

Figure 3.

Figure 3

VDE-initiated crossovers in pch2∆ mutants are partially MutLγ-dependent. (A) VDE-initiated COs in MLH3 pch2∆ and mlh3∆ pch2∆ cells. (A) CO frequencies, average signal of CO1 and CO2 for 8 and 9 h samples from two independent experiments. For inserts at CCT6, IMD3, FIR1 and RIM15, 9 h values are from a single experiment. Data for inserts at HIS4 and URA3 are from Medhi et al. (2016). (B) fraction of COs that are MutLγ-independent (ratio of CO frequencies in mlh3∆ vs. MLH3), plotted as a function of CO frequencies in MLH3 strains. CO frequencies in MLH3 pch2∆ and mlh3∆ pch2∆ differ significantly for inserts at all loci (adjusted p values ≤ 0.03) except CCT6 and RIM15 (adjusted p values of 0.06 and 0.07, respectively). (C, D) VDE-initiated NCOs, as in panels (A) and (B). NCO frequencies in MLH3 pch2∆ and mlh3∆ pch2∆ do not differ significantly for any locus (adjusted p values ≥ 0.05). Error bars in all panels denote standard deviation. See Figure S2C for summary plots with CO and NCO values for all genotypes.