Table 4. RNAi experiments expressing CG17514 RNAi-activating IRs with tub-driven GAL4 (at 29 C) suggest that CG17514 is essential. However, viable progeny emerge when using the eIF-4B RNAi line (VDRC 31364). *Escapers emerging from the VDRC 47269 CG17514 RNAi cross display a Minute phenotype and are sterile.
| Female parent | Balancer progeny | RNAi driven progeny | % of expected RNAi flies |
|---|---|---|---|
| CG17514 RNAi / CG17514 RNAi (VDRC 47269) | 195 | 17* | 9% (semi-lethal) |
| CG17514 RNAi / CG17514 RNAi (BL34355) | 303 | 0 | 0% (lethal) |
| CG17514 RNAi / CG17514 RNAi (VDRC 47268) | 151 | 105 | 63% (viable) |
| eIF-4B RNAi /eIF-4B RNAi (VDRC 31364) | 196 | 109 | 71% (viable) |
| eIF-4B RNAi /CyO (BL57305) | 324 | 123 | >100% (viable) |