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. 2019 Mar 26;9(5):1777–1783. doi: 10.1534/g3.119.400156

Figure 4.

Figure 4

Condensin hinge ts mutants with single amino acid substitutions. (A) Spot test results describing the temperature sensitivity of the 11 condensin hinge ts mutants newly isolated from the 58 mutations described in Figure 3B. 9 of the 11 condensin hinge ts mutants are strongly sensitive to high temperature, while the temperature sensitivity of cut14-L499P and cut3-S647P is weak. (B) Sensitivity of newly isolated condensin hinge ts mutants to DNA damaging agents at the permissive temperature (26°C). Among the 11 ts mutants, only cut14-L617P is sentive to damage. HU, CPT and UV designate hydroxyurea, camptothecin and ultraviolet respectively.