Figure 2.

Neighbour-joining phylogenetic tree showing the relationship between different Fth strains and the Fth-Must DNA, tularaemia outbreak, Germany, autumn 2016

The alignment was performed using progressive Mauve alignment (MUSCLE 3.6) and the phylogenetic tree was built with Geneious (Geneious 10.0.5) (Tamura–Nei, Neighbour-Joining, 100 bootstrap replicates), setting Fth OSU18 strain as outgroup. The branch labels indicate the posterior probability and the branch length corresponds to the evolutionary distance (substitutions per site). For details of canSNP analysis B.X clusters and subclusters, see Table 2 and references [26-28]. German federal states: Brandenburg (BB); Mecklenburg-Western Pomerania (MV); Lower Saxony (NI); Rhineland-Palatinate (RP). Countries: France (FR), Germany (DE), Lithuania (LT), and Sweden (SE).