Figure 3.
Detection of mammalian DNA in must samples, tularaemia outbreak, Germany, autumn 2016
M: DNA marker; NTC: negative template control; PTC: positive template control; SR: sweet reserve; YW: young wine.
Samples A-856/1–3 (lanes 1–3 and lanes 7–8) were analysed in PCR with primers detecting vertebrate cytochrome b (UNFOR403, UNREV1025, fragment size 623 bp). As a positive template control, sheep blood (PTC, lane 4) was used. Chromosomal DNA of Francisella sp. strain W12–1067 (NTC, lane 5) and water (NTC, lane 6) served as negative template controls. DNA ladder (M): GeneRuler 1 kb DNA Ladder. Sample A-856/1 = YW sort 1B, A-856/2 = YW sort 1A, A-856–3 = SR sort 1A.
