Table 4.
Dpc | 12:12 h L:D | 1:23 h L:D | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Eggs | Larvae | Protonymphs | Deutonymphs | Adults | Eggs | Larvae | Protonymphs | Deutonymphs | Adults | |
1 | 100 | 0 | 0 | 0 | 0 | 100 | 0 | 0 | 0 | 0 |
2 | 0 | 23.20 ± 8.60 | 76.80 ± 8.60 | 0 | 0 | 0 | 17.87 ± 3.40 | 82.13 ± 3.40 | 0 | 0 |
3 | 0 | 0 | 100 | 0 | 0 | 0 | 0 | 100 | 0 | 0 |
4 | 0 | 0 | 100 | 0 | 0 | 0 | 0 | 100 | 0 | 0 |
5 | 0 | 0 | 100 | 0 | 0 | 0 | 0 | 100 | 0 | 0 |
6 | 0 | 0 | 10.87 ± 6.59 | 89.13 ± 6.59 | 0 | 0 | 0 | 75.67 ± 18.54 | 24.33 ± 18.54 | 0 |
7 | 0 | 0 | 6.90 ± 4.37 | 93.10 ± 4.37 | 0 | 0 | 0 | 52.80 ± 26.17 | 47.20 ± 26.17 | 0 |
8 | 0 | 0 | 2.20 ± 2.25 | 93.73 ± 1.10 | 4.07 ± 1.22 | 0 | 0 | 23.73 ± 10.23 | 73.87 ± 8.36 | 2.40 ± 2.61 |
9 | 0.83 ± 0.06 | 0 | 0 | 78.60 ± 3.82 | 20.57 ± 3.87 | 2.70 ± 4.68 | 0 | 3.20 ± 3.87 | 77.47 ± 6.87 | 16.63 ± 3.40 |
10 | 3.33 ± 0.96 | 0 | 0 | 51.37 ± 9.50 | 45.30 ± 8.55 | 7.83 ± 3.51 | 0 | 0 | 58.53 ± 10.02 | 33.63 ± 10.75 |
Notes: The complete life-cycle of D. gallinae, from the egg to a new egg generation, was carried out using the in vivo feeding system. The initial cohort size was 300 eggs. The observations took place every 24 h for 10 days. At each observation, the number of mites at different stages was counted under a stereomicroscope and the rate of mites at different stages was calculated every day. Observations were continued until the mites had reached the adult stage and laid the first egg