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. 2019 May 7;19:89. doi: 10.1186/s12866-019-1461-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of the NK production mediated by different triple-promoter systems. a Fibrinolytic activities of NK in the supernatant. b SDS-PAGE analysis of the culture supernatant. Recombinant strains promoted by different triple-promoters were cultured in the TB medium for 36 h, and then cells and the supernatant culture were separated by centrifugation. Lane 1–8: P_P43-P_P43-P_HpaII, P_P43-P_HpaII-P_HpaII, P_HpaII-P_P43-P_P43, P_P43-P_HpaII-P_P43, P_P43-P_P43-P_P43, P_HpaII-P_HpaII-P_P43, P_HpaII-P_P43-P_HpaII, and P_HpaII-P_HpaII-P_HpaII; Lane M: standard marker proteins. The arrow indicates NK bands