Fig. 5.

—Intergenic mutations with functional effects on transcription. Luminescence from transcriptional lux reporter fusions with mutated and wild-type alleles was measured at OD₆₀₀=0.15 and normalized by cell density. Transcriptional fusions were examined in Luria-Bertani (LB) and ABTGC minimal media. Normalized mean luminescence was calculated for three biological replicates, followed by calculating the relative fold change of mutant versus wild-type allele. Statistical analysis of the difference between two means was performed by a two-tailed Student’s t test and the asterisk denotes P < 0.05.