Skip to main content
. 2019 Apr 13;11(5):1385–1397. doi: 10.1093/gbe/evz083

Fig. 6.

Fig. 6.

—Mutations in the intergenic region between ampC and ampR cause an increased tolerance towards imipenem and ampicillin. The values for minimal inhibitory concentration (MIC) are shown on the y axis and the constructed mutations in each strain of PAO1 are displayed under the y axis. Mutation G-98A upstream of ampC derives from isolate DK2-CF173-1995. Three mutations G-38A, C-66T and G-78A upstream of ampC originate from isolate DK1-P43-M2-2002. An SNP mutation at the start of ampC (G7A) in DK1-P43-M2-2002 was also constructed in laboratory strain PAO1 to isolate the effect of this mutation and the effect of intergenic mutations from DK1-P43-M2-2002. Error bars indicate standard deviation from three different biological replicates. Double asterisk indicates statistically significant difference between mean MIC of the strains (two-tailed Student’s t test, P < 0.01).