Fig. 5.
Deletion of exons 8-34 in the dystrophin gene results in the absence of dystrophin protein in the sarcolemma of muscle cells. Cross-sections through the TA muscle of mdx and DMDdel8-34 mutant male mice, as well as those of their respective wild-type control C57BL/10ScSnJ (B10) and CBA×C57BL/6 mice, were immunostained with an antibody against the C-terminal amino acids of dystrophin (ab15227) and an Alexa Fluor 488 secondary antibody (green channel). The absence of dystrophin is evident in both DMDdel8-34 and mdx mutant mice. Scale bars: 75 µm.