Fig. 7.

Analysis of DAGC component expression levels in the muscles of DMD^del8-34 mice in comparison with that in wild-type controls. (A) Cross-sections through the TA muscle of DMD^del8-34 mutant male mice, as well as those of their wild-type control CBA×C57BL/6 mice, were immunostained with an antibody against DAGC proteins (green). Nuclei were counterstained with To-Pro-3 Iodide (pseudocoloured magenta). Scale bars: 100 µm. (B) Total muscle lysates of TA muscles from DMD^del8-34 and CBA×C57BL/6 mice were analyzed by western blot using an antibody against DAGC proteins. Alpha-actin was used as a loading control. Representative images of three independent experiments are shown. Graphical representation of DAGC component expression in TA muscle of DMD^del8-34 in comparison with that in wild-type control mice from blots is shown to the right. Downregulation of alpha-sarcoglycan and beta-dystroglycan, but upregulation of beta-sarcoglycan and syntrophin total protein level are shown. Bar charts show the mean±2 s.e.m.