Fig. 4.

FOXO regulates AKT activity downstream of fs(1)h. (A) Western blot analysis of AKT Ser505 phosphorylation in control and fs(1)h fat-body-knockdown flies, either wild-type or heterozygous for foxo. Values represented as intensity relative to α-tubulin, shown as mean. (B) Western blot analysis of total and cleaved REL abundance in control and fs(1)h fat-body-knockdown flies, either wild-type or heterozygous for foxo. Values represented as intensity relative to α-tubulin, shown as mean+s.e.m.; genotypes were compared using unpaired two-tailed t-test. (C) foxo mRNA expression in control and fs(1)h fat-body-knockdown flies, either wild-type or heterozygous for foxo. Normalised to expression of α-tubulin as a loading control. Values shown as mean+s.e.m.; genotypes were compared using unpaired two-tailed t-test. (D) fs(1)h expression in flies wild-type, heterozygous or homozygous for foxo mutation. Normalised to expression of α-tubulin as a loading control. Values shown as mean+s.e.m.; genotypes were compared using unpaired two-tailed t-test. Throughout, **P<0.01, ***P<0.001, ****P<0.0001.