Fig. 4.

Chronic Co²⁺ treatment increases TRP channel expression in the heart. a Representative immunoblots of whole cardiac left ventricular homogenates (20 µg) from control and 7-day or 28-day Co²⁺-treated animals. Blots were probed for either DMT1, TRPC6 and TRPM7 and protein expression quantified using GAPDH as an internal control. b Histograms showing mean data presented as ratios of DMT1:GAPDH, TRPC6:GAPDH, or TRPM7:GAPDH from control (n = 3) and Co²⁺-treated (n = 6) groups following 7 days and 28 days of treatment. Data are presented as means ± S.E.M. and statistical analysis performed using two-sample t-tests (n ≥ 3, *p < 0.05, treatment group vs. control group)