Table 2.
Primers used for RT-qPCR
| Primers | GenBank number | Primer sequence | Amplicon length |
|---|---|---|---|
| ACTB F | NM_001101.3 | GCTCACCATGGATGATGATATCGC | 169 |
| ACTB R | CACATAGGAATCCTTCTGACCCAT | ||
| CAPN10 F | NM_023083.3 | TCTCACCGGGCTACTACCTG | 86 |
| CAPN10 R | CCCGGTAGAGAAGACTCGGA | ||
| CCNG1 F | NM_004060.3 | GCCTCTCGGATCTGATATCGT | 138 |
| CCNG1 R | CATTCAGCTGGTGTAGCAGT | ||
| DCX F | NM_000555.3 | GGAAAGGGTTTGATGAATAGCACAA | 122 |
| DCX R | AAGCCCTCTTTCCTACTCTAATGTG | ||
| EEF1A1 F | NM_001402.5 | TGTTCCTTTGGTCAACACCGA | 122 |
| EEF1A1 R | ACAACCCTATTCTCCACCCA | ||
| EID2 F | NM_153232.3 | GGCATCGCTCTGTCCAGTTA | 74 |
| EID2 R | GCTTGGACATCTCAGACCGT | ||
| GAPDH F | NM_002046.5 | GTTCGACAGTCAGCCGCATC | 90 |
| GAPDH R | TCCGTTGACTCCGACCTTCA | ||
| GFAP F | NM_002055.4 | GTGAAGACCGTGGAGATGCG | 76 |
| GFAP R | TGCCTCACATCACATCCTTGT | ||
| HPRT1 F | NM_000194.2 | AGGCGAACCTCTCGGCTTTC | 166 |
| HPRT1 R | CTGGTTCATCATCACTAATCACGAC | ||
| MAP2 F | NM_002374.3 | TGCCTCAGAACAGACTGTCAC | 101 |
| MAP2 R | AAGGCTCAGCTGTAGAGGGA | ||
| MYC F | NM_002467.4 | CCCTCCACTCGGAAGGACTA | 96 |
| MYC R | GCTGGTGCATTTTCGGTTGT | ||
| NAT1 F | NM_001160170.3 | TGGTTGCCGGCTGAAATAAC | 93 |
| NAT1 R | TCTGTCTAGGCCAGTCTCCT | ||
| NRF1 F | NM_001001928.2 | CAGCCGCTCTGAGAACTTCAT | 148 |
| NRF1 R | GTCTTCATCAGCACTCAGCATACTA | ||
| PHB F | NM_001281496.1 | TGGAAGCAGGTGAGAATGGAG | 76 |
| PHB R | ATCATGGAGCAGAGGAGGACT | ||
| PPARGC1A F | NM_003201.2 | TAGTAAGACAGGTGCCTTCAGTTC | 174 |
| PPARGC1A R | CTCGATGTCACTCCATACAGACTC | ||
| RABEP2 F | NM_024816.2 | AGGAAGGGGCAAATGGTGAG | 96 |
| RABEP2 R | CAGCCTTCATGGTTTCCATTTCTG | ||
| RPLP0 F | NM_001002.3 | CCTCGTGGAAGTGACATCGT | 76 |
| RPLP0 R | CTGTCTTCCCTGGGCATCAC | ||
| S100B F | NM_006272.2 | CGGAGGGAACCCTGACTACA | 132 |
| S100B R | TCTGCATGGATGAGGAACGC | ||
| TBP F | NM_003194.4 | GCAAGGGTTTCTGGTTTGCC | 80 |
| TBP R | CAAGCCCTGAGCGTAAGGTG | ||
| TFAM F | NM_005011.4 | TGAAAGATTCCAAGAAGCTAAGGGT | 132 |
| TFAM R | TAACGAGTTTCGTCCTCTTTAGCAT | ||
| TUBB3 F | NM_006086.3 | CAACCAGATCGGGGCCAAGTT | 146 |
| TUBB3 R | GAGGCACGTACTTGTGAGAAGA | ||
| UBC F | NM_021009.6 | ACGGGACTTGGGTGACTCTA | 82 |
| UBC R | ATCGCCGAGAAGGGACTACT | ||
| ZNF324B F | NM_207395.2 | CATTGGAAGGACAAACCTAGGATGATG | 164 |
| ZNF324B R | CTTATCTGCTCCAAAGCTATCACTGTC |
F forward, R reverse