Table 2.

Primers used in this study

Primer ID	Sequence	Endonuclease/template
Pantothenate
panBfwd	tatacatatgaaaaataccactgaaac	NdeI/C. acetobutylicum
panCfwd	tatacatatggaaattttgcattcc	NdeI/C. acetobutylicum
panDfwd	tatacatatgcatttaaatatgttaaaatctaaa	NdeI/C. acetobutylicum
panBrev	tatagctagcttatttggtaatttcgtctataatg	NheI/C. acetobutylicum
panCrev	tatagctagcttatatattccaagtgaaattatctataagg	NheI/C. acetobutylicum
panDrev	Tatagctagcttagccaattgtcc	NheI/C. acetobutylicum
panB2fwd	Tataactagtatgaaaaataccactgaaac	SpeI/C. acetobutylicum
Biotin
bioYDAF1	tatacatatgattatggtttctatttttgc	NdeI/C. acetobutylicum
bioYDAR1	tatagctagcttattcaagatactgctctatactttc	NheI/C. acetobutylicum
bioBDFF1	tatacatatgagcaatattttgaaacatatcg	NdeI/D. carboxidivorans
bioBDFR1	tatagctagcttaaatcaatcccagttc	NheI/D. carboxidivorans
bioHCAF1	tatacatatgttggaaaaacagagattaatcc	NdeI/D. carboxidivorans
bioHCAR1	tatagctagcttaatctattccaaaatagttattaattgc	NheI/D. carboxidivorans
Thiamine
thiCpurFfwd	gcagggcccaatacgattatctcctttc	ApaI/C. ragsdalei
thiCpurFrev	gcatcctgcaggtaaattttgttcttcatt	SbfI/C. ragsdalei
ACE
SHAF	tccgcggaagggcattttctactttcttc	SacII/C. ljungdahlii
SHARd	agcggccgccacatcttcagaaattataattttttctcc	NotI/C. ljungdahlii
SHARc	agcggccgcttatttgaatactctacttccaggc	NotI/C. ljungdahlii
GHAF	tatgctagcgtcgaaaaaatcaatgcacgatgc	NheI/C. ljungdahlii
GHAR	aggcgcgcctactgggtaacggaacctg	AscI/C. ljungdahlii
CH21thlF1	tatagctagcccgctttttaacaaaatatattg	NheI/pMTL8xxx2
CH21thlR1	tataactagtggggtaacagataaaccatttc	SpeI/pMTL8xxx2
cacpyreF	atggaacaatacaaacaagaatttatag	/ C. acetobutylicum
cacpyreR	gagtactataaagagtatggagtaaaatag	/ C. acetobutylicum
HiFi assembly
P1F	atgaaatagattgaaatgagcaatattttgaaacatatc	/ pMTL-FJAL22
P2R	gctactacacttaaatcaatcccagttcttttc	/ pMTL-FJAL22
P3F	ggattgatttaagtgtagtagcctgtgaaataag	/ pMTL-FJAL23
P4R	ttgattttttcgacgctagcttaatctattccaaaatagttattaattgc	/ pMTL-FJAL23