Table 3.
Plasmids constructed in this study
| Plasmid | Backbone a | Functional module | F-primer | R-primer |
|---|---|---|---|---|
| ACE | ||||
| pANNE98 | pMTL84141 | dSHA | SHAF | SHARd |
| pANNE99 | pMTL84141 | GHA | GHAF | GHAR |
| pMTL-AMH100 | pANNE99 | ACE PyrE deletion | ||
| pMTL-AMH101 | pMTL-CH14 | ACE PyrE deletion | ||
| pMTL-AMH102 | pMTL84141 | ACE PyrE wt-restore | SHAF | GHAR |
| pMTL-AMH103 | pANNE99 | ACE PyrE correction | SHAF | SHARc |
| pMTL-CH14 | pMTL84151 | C. acetobutylicum pyrE b | cacpyreF | cacpyreR |
| pMTL-CH20 | pMTL84151 | ACE PyrE correction | ||
| pMTL-CH21 | pMTL-CH20 | ACE PyrE correction + BB2 | ||
| pMTL-CH23 | pMTL-CH21 | ACE PyrE correction + Pthl + BB2 | CH21thlF1 | CH21thlR1 |
| Pantothenate | ||||
| pMTL-FJAL3 | pMTL82152 | Pthl-panBCD | panBfwd | panDrev |
| pMTL-FJAL4 | pMTL83153 | Pfdx-panCD | panCfwd | panDrev |
| pMTL-FJAL5 | pMTL83153 | Pfdx-panBC | panBfwd | panCrev |
| pMTL-FJAL6 | pMTL82252 | Pthl-panB | panBfwd | panBrev |
| pMTL-FJAL7 | pMTL82252 | Pthl-panC | panCfwd | panCrev |
| pMTL-FJAL8 | pMTL82252 | Pthl-panD | panDfwd | panDrev |
| pMTL-FJAL10 | pMTL-CH23 | Pthl-panBCD | panB2fwd | panDrev |
| Biotin | ||||
| pMTL-FJAL20 | pMTL82252 | Pthl-bioYDA | bioYDAF1 | bioYDAR1 |
| pMTL-FJAL21 | pMTL82252 | Pthl-bioBDF | bioBDFF1 | bioBDFR1 |
| pMTL-FJAL22 | pMTL83153 | Pfdx-bioHCA | bioHCAF1 | bioHCAR1 |
| pMTL-FJAL23 | pMTL-CH23 | Pthl-bioBDF-Pfdx-bioHCA | HiFi assembly | |
| pMTL-FJAL24 | pMTL83251 | Pthl-bioBDF-Pfdx-bioHCA | ||
| Thiamine | ||||
| pMTL-BAS1 | pMTL85242 | Pnative-thiC-purF | thiCpurFfwd | thiCpurFrev |
apMTL8xxxx vectors described by Heap et al. 2009
bContains C. acetobutylicum pyrE gene in backbone downstream of catP