Fig. 4.

Alteration in myelin gene expression after in vitro mechanical tensile strain of 20% and 30%. Total RNA was extracted and RT-qPCR was performed in control (Ctrl) and stretched (Stretch) cells for myelin genes Mag (a, d), Cnp (b, e), and Plp (c, f, g, i) after strain of 20% (a, b, c, g) and 30% (d, e, f, i). Results represent the mean ± SEM of three independent experiments performed in triplicate. *p < 0.05; **p < 0.01. (h, j) Proteins were extracted from control (Ctrl) and stretched (Stretch) 158N cells and western blot was performed for myelin protein PLP (26 kDa) following 20% (h) and 30% (j) of strain. β-Actin was used as a loading control. Results represent the mean ± SEM of three independent experiments