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. 2019 Apr 30;11(5):e9960. doi: 10.15252/emmm.201809960

Figure 2. TGF‐β–induced mesenchymal phenotype increases sensitivity to Plk1 inhibition.

Figure 2

  • A
    Three epithelial/resistant non–small‐cell lung cancer cell lines were treated with 5 ng/ml TGF‐β for 14 days to induce a mesenchymal phenotype, which was confirmed with Western blot (left) and qPCR (right) analysis of epithelial‐to‐mesenchymal transition (EMT) markers.
  • B
    Parental and TGF‐β isogenic cell lines were treated with 50 nM volasertib for 72 h. Cells were then harvested, and lysates were immunoblotted for cleaved PARP and γH2AX proteins that were subsequently quantitated and normalized with β‐actin.
  • C, D
    Apoptosis was measured by the Apo‐BrdU assay (C), and DNA damage was measured by the Comet assay (D).
Data information: Data are means ± standard error of the mean from three independent experiments. Significant differences using two‐way analysis of variance with Bonferroni or Benjamini–Hochberg correction for multiple comparison are indicated (*P < 0.01).Source data are available online for this figure.