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. 2019 Apr 8;103(11):4253–4268. doi: 10.1007/s00253-019-09717-y

Table 1.

Reported PET hydrolases and their homologs

Name Source GenBank accession Sequence identity (%) References PDB ID References
Lipase Streptomyces exfoliatus 62.8 Wei et al. (1998) 1JFR
BTA-1 (TfH)* Thermobifida fusca DSM43793 AJ810119.1 100 Müller et al. (2005); Kleeberg et al. (1998, 2005); Dresler et al. (2006)
BTA-2 92.3
Tfu_0882 Thermobifida fusca YX (T. fusca WSH03-11) AAZ54920.1 93.1 Lykidis et al. (2007); Chen et al. (2008); Su et al. (2013)
Tfu_0883* AAZ54921.1 100
TfCut1 Thermobifida fusca KW3 CBY05529.1 94.3 Herrero Acero et al. (2011)
TfCut2* CBY05530.1 99.1 4CG1, 4CG2, 4CG3 Roth et al. (2014)
Est1* Thermobifida alba AHK119 BAI99230.2 83.1 Hu et al. (2010)
Est119 BAK48590.1 82.4 Thumarat et al. (2012) 3VIS, 3WYN6AID Kitadokoro et al. (2012); Kawai et al. (2013); Kitadokoro et al. (2019)
Thc_Cut1 Thermobifida cellulosilytica DSM44535 ADV92526.1 100 Herrero Acero et al. (2011) 5LUI, 5LUJ, 5LUK, 5LUL Ribitsch et al. (2017)
Thc_Cut2* ADV92527.1 93.1
Thf42_Cut1 Thermobifida fusca DSM44342 ADV92528.1 97.7 Herrero Acero et al. (2011)
Tha_Cut1 Thermobifida alba DSM43185 ADV92525.1 98.5 Ribitsch et al. (2012b)
Thh_Est Thermobifida halotolerans DSM44931 AFA45122.1 75.1 Ribitsch et al. (2012)
TfAXE Thermobifida fusca NTU22 ADM47605.1 98.9 Huang et al. (2010)
LC-cutinase Metagenome from leaf-branch compost AEV21261.1 56.6 Sulaiman et al. (2012) 4EB0 Sulaiman et al. (2014)
Cut1 Thermobifida fusca NRRL B-8184 JN129499.1 93.1 Hegde and Veeranki (2013)
Cut2 JN129500.1 100
Tcur1278 Thermomonospora curvata DSM43183 ACY96861.1 61.9 Chertkov et al. (2011); Wei et al. (2014b)
Tcur0390* ACY95991.1 61.9
Cut190 Saccharomonospora viridis AHK190 AB728484 65.6 Kawai et al. (2014)
Cut190_S226P 4WFI,4WFJ, 4WFK Miyakawa et al. (2015)
Cut190_S226P_R228S (Cut190*) Oda et al. (2018) 5ZNO, 5ZRQ, 5ZRR, 5ZRS Numoto et al. (2018)
PETase Ideonella sakaiensis strain 201-F6 GAP38373.1 51.7 Yoshida et al. (2016) 5XG0, 5XFY, 5XFZ, 5XH2, and 5XH3
5XJH and 5YNS
6ANE 6EQD, 6EQE, 6EQF, 6EQG, 6EQH
Han et al. (2018); Joo et al. (2018) Fecker et al. (2018); Austin et al. (2018)
Cbotu_EstA* Clostridium botulinum ATCC3502 KP859619 5AH1 Perz et al. (2016); Biundo et al. (2018a)
Cbotu_EstB KP859620

The sequence identities were calculated based on the pairwise alignments of the mature protein sequences of each enzyme with the first described synthetic polyester hydrolase BTA-1 (TfH) from T. fusca DSM43793

*Higher activity than the other enzymes