Figure 2. a–c.

miR-155 was up regulated in both liver and spleen in ALF

The expression of hepatic miR-155 was detected in liver tissue of mice induced by D-GalN/LPS, D-GalN, LPS and saline treatment by RT-PCR respectively (A). There are four groups for this experiment (D-GalN/LPS, D-GalN, LPS and saline), with 6 time points (0, 1, 3, 5, 7, and 9 h) for each group. There were 10 mice per time point for D-GalN, LPS and saline groups respectively and with 100% survival rate for all of the three groups. In D-GalN/LPS group, there were 10, 10, 10, 12, 20 and 35 mice for each time point (0, 1, 3, 5, 7, and 9 h), respectively, prior to challenge. The survival rate was 100% (0 h), 100% (1 h), 100% (3 h), 90% (5 h), 50% (7 h) and 30% (9 h) in D-GalN/LPS challenge group. In addition, the miR-155 PCR productions with D-GalN/LPS induction for 7 h was used to run the gel, U6 was used as the internal control (B). miR-155 expression was detected in spleen tissue in mice with D-GalN/LPS induction for 7 h (C) (n = 10).