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Hep1-6 cells were transfected with miR-155 inhibitor (200nM) or mimic (100nM) (A), dye3 (red fluorescence) was used as positive control (B), the transfection efficiency was verified by RT-PCR. The expression of TNF mRNA in the Hepa1-6 cells transfection of inhibitor of miR-155 (anti-155) or mimic of miR-155 (155-mimic), followed by stimulation of D-GalN/TNF for another 48h, were detected using RT-PCR (C).
