Fig. 1.

Alterations of CRMP2 phosphorylation levels in a rodent model of spared nerve injury (SNI). (A) Amino acid sequence of rat CRMP2 (Universal Protein Resource #P47942 (rat)) identifying the kinases (Src-family kinase Fyn (blue), glycogen synthase kinase 3β (GSK3β; green), cyclin-dependent kinase 5 (Cdk5; red), and Rho-associated protein kinase (ROCK; orange) and their target phosphorylated sites (numbers refer to the amino acid modified by the kinases). Representative immunoblots of spinal cord dorsal horn (B) or dorsal root ganglia (D) from spared nerve injury (SNI) rodents, ipsilateral (injured) and contralateral (non-injured) sides (n = 7), probed with phosphorylation-specific CRMP2 antibodies. No signals were detected for CRMP2 phosphorylated by the Src-family kinase Yes (Y479). Quantitative analyses of CRMP2 phosphorylation levels in ipsilateral (injured) and contralateral (non-injured) sides from SNI rodents (C, E, G, I). Tubulin was used as a loading control. Data show means ± s.e.m. of seven animals. *p < 0.05; Kruskal-Wallis test with Dunnett’s post hoc comparisons. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)