Fig. 3.

SNI mediated allodynia requires CRMP2 expression. (A) Representative immunoblot and bar graph showing decreased CRMP2 expression in DRGs, 24 h after CRMP2 siRNA intrathecal injection. Data is shown as mean ± SEM, n = 6, *p < 0.05, Mann-Whitney compared to control siRNA. (B) Rats developed allodynia 7 days after SNI. Intrathecal injection (black arrow) of CRMP2 or Control siRNAs significantly reversed the mechanical allodynia, measured by paw withdrawal threshold, at 3 and 24 h after injection (n = 6; *p < 0.05 vs. pre-injection baseline, 2-way ANOVA, post hoc Student-Neuman-Keuls). (C) Summary of data shown in panel (B) as area under the curve (AUC) for 0–48 h. Mean ± s.e.m., n = 6, *p < 0.05 versus control siRNA, Mann-Whitney.