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Phylogenetic tree including next-generation sequencing results for cases 4 (Bighorn no. 4), 6 (Bighorn no. 6), and BTV-10 vaccine (vaccine). Neighbor-joining tree based on HKY genetic distance of near full-length (open reading frame: 11,696 bp) bovine viral diarrhea virus (BVDV) sequences with 1,000 bootstrapping replicates. Nodes with >70% support are shown. The vaccine strain and bighorn samples cluster together and with other BVDV-1b subgenotypes.
