Figure 1.

Oligonucleotide analysis of the canine-specific endogenous internal positive control (EIPC-K9) assay in respect to homologous and heterologous species’ mitochondrial DNA target sequences. The second column indicates qualitative results (positive or negative) in respect to EIPC-K9 specificity to 2 independent blood samples from homologous and heterologous species (dog, gray wolf, coyote, horse, cat, goat, sheep, white-tailed deer, skunk, cow, pig, chicken, raccoon, and red fox). Mismatch analysis of base-pair positions that are not conserved against EIPC-K9 primers and probe are highlighted in gray.