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. 2019 May 8;17(5):e3000254. doi: 10.1371/journal.pbio.3000254

Fig 3. ABC169 supports the maturation of immature female schistosomes following pairing with a male worm.

Fig 3

(A) Vitellaria development visualized by Fast Blue BB labeling in immature female schistosomes in the presence or absence of male worms in BM169 or ABC169 at D20 of culture. Black pigment in ABC169 parasites represents digested RBCs. (B) Eggs laid by immature female in BM169 or ABC169 on day 20 after pairing with a male. (C) Proportion of females laying eggs (left axis, magenta) and number of eggs laid per worm pair (right axis, green) from D1 to D20 following pairing of immature females with male worms in ABC169. n = 31 worm pairs examined in 4 separate experiments. Error bars represent 95% confidence intervals. Underlying primary data can be found in S1 Data. (D) Eggs laid by previously immature females commence embryonic development. Left, representative developing EdU-labeled embryo from egg laid in ABC169 on D20. Scale bars: A, B, 100 μm; D, 20 μm. AB169, Ascorbic Acid, Blood Cells, Cholesterol, and BM169; BM169, Basch’s medium 169; D, day; EdU, 5-ethynyl-2′-deoxyuridine; RBC, red blood cell.