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. 2019 May 8;17(5):e3000254. doi: 10.1371/journal.pbio.3000254

Fig 5. Smp_248100 is expressed in the primordial vitellaria.

Fig 5

(A) A schematic diagram of protein product encoded by Smp_248100. This protein contains a ZnF-C4 domain similar to other NR proteins. (B) Whole-mount in situ hybridization showing expression of Smp_248100 in a mature female (left), immature female (middle), or male worm (right). Smp_248100 highly expressed in female worms exclusively in the region of vitellaria. Representative images from 3 experiments with n > 35 parasites. Scale bar, 100 μm. (C–D) Two-color FISH for H2B (green) and Smp_248100 (magenta) mRNAs on (C) immature females or (D) sexually mature females. In immature virgin females, Smp_248100 was only expressed in H2B+ cells. In adult females, Smp_248100 was expressed in H2B+ cells as well as some H2B– cells. We did not detect Smp_248100 expression in mature vitellocytes in the vitelline duct (bounded by dashed yellow lines). Representative images from n > 30 parasites examined in 3 separate experiments. Scale bars: B, 100 μm; C, 50 μm. DIC, differential interference contrast; FISH, fluorescent in situ hybridization; H2B, Histone H2B; NR, nuclear receptor; ZnF-C4, Zinc finger, C4 type.