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. 2019 May 8;14(5):e0216056. doi: 10.1371/journal.pone.0216056

Fig 4. Validating a method to measure "outside-in" leak in vivo.

Fig 4

C57BL/6 wild-type mice were challenged with saline vehicle or 10 μg polyI:C (pIC) for three days. Twenty-three hours after the final challenge, 0.2 mg 4kDa FITC-dextran was instilled oropharyngeally into the airway. One hour later, mice were euthanized and BAL fluids, BAL cell pellets, blood and lung tissue were collected. FITC-dextran levels were determined by fluorimetry using a standard curve to calculate total microgram amounts. Data shown are mean±SD from (A) BAL fluids, (B) serum, (C) BAL cell pellets, (D) lung tissue supernatants, and (E) lung cell pellets. BALF, serum, lung homogenate, and lysate from lung cell pellet were analyzed for. Data are mean ± SD pooled from two independent experiments (male and female). Asterisks indicate **p<0.01 and *p<0.05 using unpaired Student’s t-test.