Fig 1. A. fumigatus supernatant effects on P. aeruginosa growth and pyoverdine production.

Planktonic 10AF supernatant (10AFsup) was diluted in RPMI from 50% to 0% 10AFsup, and incubated with P. aeruginosa cells (5x10⁷/ml) at 37°C for 24h. Relative pyoverdine (PYOV) concentrations (A) were calculated using the quotient A405 (PYOV)/A600 (bacterial growth: B). Supernatants shown in A and B, as well as PA14 supernatant not containing 10Afsup, were compared with respect to their activities on 10AF forming biofilm (C: BCAM) or preformed biofilm (D: BHAM) metabolism. E: 10AF forming biofilm was incubated with 10 or 25% PA14 wildtype supernatant with or without the addition of 25% 10AFsup for 24 hours. Effects on 10AF forming biofilm metabolism were evaluated by XTT assay. F: 10AF forming biofilm was incubated with 5 or 10 μM pyoverdine (PYOV) with or without the addition of 25% 10AFsup for 24 hours. Effects on 10AF forming biofilm metabolism were evaluated by XTT assay. Statistics for A and B: 1way ANOVA, RPMI (white bar) vs. all groups containing Afsup (black bars). Statistics for C and D: 1way ANOVA, PA14 supernatant (white striped bar) vs. PA14 supernatant containing 10AFsup (grey striped bars). Other comparisons by t-Test as indicated by the ends of the brackets. Statistics for E and F: t-Test, RPMI (white bar) vs. all other bars. Other comparisons as indicated by the ends of the brackets. One, two or three asterisks = p ≤ 0.05, p ≤ 0.01 or p ≤ 0.001, respectively.