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. 2019 May 8;14(5):e0216085. doi: 10.1371/journal.pone.0216085

Fig 4. AfΔsidA is more sensitive towards PA14 or pure pyoverdine than its wildtype.

Fig 4

A: Mixtures (25%) of freshly prepared AF13073, AfΔsidA, AF46645, AfΔsidC, AfΔsidF, or AfS77 supernatants were combined with pyoverdine [10 μM], and tested for effects on 10AF forming biofilm metabolism. Fungal metabolism was measured by XTT assay. Measurements for controls (no pyoverdine) in each group were regarded as 100%. Statistics: t-Test, for each group: no pyoverdine (grey bar) vs. pyoverdine (black bar). Other comparison as indicated by the ends of the bracket. B: AF13073, AfΔsidA, AF46645, AfΔsidC, AfΔsidF or AfS77 BCAM assays were incubated with either RPMI, PA14 supernatant, or 5 μM pyoverdine. Fungal metabolism was measured by XTT assay. For each fungus RPMI control measurements were regarded as 100%. Statistics: t-Test, comparison: RPMI (white bars) vs. PA14 supernatant (grey bars), or pyoverdine (black bars) for each fungus. Other comparisons as indicated by the ends of the brackets. C: A 10AF BCAM assay was incubated with either RPMI, pyoverdine [10 μM], TAFC [5 or 10 μM], DF-TAFC [5 or 10 μM], or combinations of pyoverdine and TAFC or DF-TAFC. Fungal metabolism was measured by XTT assay. RPMI control measurements were regarded as 100%. Statistics: t-Test, comparison: RPMI (white bar) vs. all other bars. Other comparisons as indicated by the ends of the brackets. One, two or three asterisks = p ≤ 0.05, p ≤ 0.01 or p ≤ 0.001, respectively.