Fig 5. Absence of hydroxamate siderophores sensitizes A. fumigatus.

A: RPMI was inoculated with PA14 [5x10⁷ cells/ml], incubated for 24 hours, and the culture supernatant was sterile filtered. Growth of point inoculated AF13073 or AfΔsidA (10⁴conidia) on 3 ml solid minimal medium in the presence of 1 μM FeSO₄ plus 50–600 μl of the sterile filtered supernatants was compared after incubation for 48 h at 37°C. B: AfΔsidA (white bars) or AF13073 (black bars) BCAM assays were incubated with either RPMI or different concentrations of pyoverdine. Fungal metabolism was measured by XTT assay. Statistics: t-Test. For each fungus RPMI controls were regarded as 100%. RPMI controls for each fungus vs. all pyoverdine concentration. Other comparisons as indicated by the ends of the brackets. C: Wildtype (AF13073) or AfΔsidA forming biofilms were incubated with DFP [0.125–2 mM] at 37°C for 24 hour. Fungal metabolism was measured by XTT assay. Statistics: t-Test, RPMI vs. all other bars of the same group. Other comparisons as indicated by the ends of the brackets. D: Wildtype (AF13073) or AfΔsidA forming biofilms were incubated with AmB [19–312 ng/ml] at 37°C for 24 hour. Fungal metabolism was measured by XTT assay. Statistics: t-Test, RPMI vs. all other bars of the same group. Other comparisons as indicated by the ends of the brackets. One, two or three asterisks = p ≤ 0.05, p ≤ 0.01 or p ≤ 0.001, respectively.