Table 1. Primers for qRT-PCR.
| Gene symbol | Sequence accession # | Orientation | Sequence [5’ to 3’] |
Amplicon length [bp] |
|---|---|---|---|---|
| CASP3 | NM_004346.3 | forward | CATTGAGACAGACAGTGG | 108 |
| reverse | TCGCCAAGAATAATAACCAG | |||
| CASP4 | NM_001225.3 | forward | GTTTGACCATCTGCCTCC | 126 |
| reverse | CGCTGACTCCATATCCCT | |||
| CASP5 | NM_004347.3 | forward | CTTTCTGTTCTTCAACACCA | 143 |
| reverse | ATGATTTCTGTACCTTCCGA | |||
| CASP8 | NM_001228.4 | forward | CTGATTCAGAGGAGCAACCC | 200 |
| reverse | GAATATCATCGCCTCGAGGAC | |||
| CASP9 | NM_001229.4 | forward | CCATATCTAGTTTGCCCACAC | 183 |
| reverse | GAAACAGCATTAGCGACCCT | |||
| CXCL8 | NM_000584.3 | forward | CAGTGCATAAAGACATACTCC | 198 |
| reverse | TTTATGAATTCTCAGCCCTC | |||
| GAPDH | NM_002046.5 | forward | CCATGGAGAAGGCTGGGG | 195 |
| reverse | CAAAGTTGTCATGGATGACC | |||
| IL1B | NM_000576.2 | forward | TTCATTGCTCAAGTGTCTG | 128 |
| reverse | GCACTTCATCTGTTTAGGG | |||
| IL6 | NM_000600.4 | forward | AACAAATTCGGTACATCCTC | 167 |
| reverse | AAGTCTCCTCATTGAATCCA | |||
| TNF | NM_000594.3 | forward | CAGCCTCTTCTCCTTCCT | 188 |
| reverse | GGGTTTGCTACAACATGG |