Fig 2. Fly genetics and computational chemistry discovery platform.
Key steps include (A) determining suppressors and enhancers in a dominant modifier genetic screening and their in silico modelability, (B) generating DFG-out kinase models using DFGmodel, (C) virtual screenings of compound libraries against the modeled suppressors and enhancers, combining top-ranking screening results into consensus result, (D) testing top-ranking compounds for rescue of lethality (left panel) and migration of transformed cells in developing wing discs of ptc>dRetM955T flies (right panel), and (E) refining hits by combining structural elements of computationally derived hits and that of drugs and evaluating new targets.