Figure 3. Scn1a^ΔE26 mice show reduced respiratory output under control conditions and during exposure to high CO₂.

For these experiments Scn1a^fl/+ and Slc32a1^cre/+ were used as control. (A) traces of respiratory activity from a control and Scn1a^ΔE26 mouse during exposure to room air, 100% O₂ and 3–7% CO₂ (balance O₂). (B–D), summary data (n = 22 control; n = 17 Scn1a^ΔE26) show respiratory frequency (B), tidal volume (C) and minute ventilation (D) are reduced in Scn1a^ΔE26 mice compared to control under room air conditions. (E), traces of respiratory activity (left) and summary data (right) show that under room air conditions both control and Scn1a^ΔE26 mice exhibit periods of apnea; the frequency of these events were similar between genotypes, however, they lasted for a longer duration in Scn1a^ΔE26 mice compared to control. F-H (Figure 3—source data 1), summary data shows the respiratory frequency (F), tidal volume (G) and minute ventilation response of control and Scn1a^ΔE26 mice to graded increases in CO₂ (balance O₂). Scn1a^ΔE26 mice showed a blunted respiratory frequency to 5% and 7% CO₂ which resulted in a diminished CO₂/H⁺-dependent increase in minute ventilation. These results were compared using either unpaired t test (panels B-E) or two-way ANOVA followed by the Holm-Sidak multiple comparison test (panels F-H). *, difference between means p<0.05, ^#, different interaction factor, p<0.05.