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. 2019 May 8;8:e45542. doi: 10.7554/eLife.45542

Figure 5. Stabilizing beta-catenin in endothelial cells corrects the retinal vascular defect caused by loss of Dlg1.

(A–D) P7 retinas from the indicated genotypes, with the boxed region in column 1 shown at higher magnification in columns 2 and 3, and the boxed region in column 4 shown at higher magnification in column 5. Mice were injected IP with 2–3 mg of sulfo-NHS-biotin 1–2 hr before sacrifice. Dashed white lines indicate the edge of the retina. (E–H) Quantification of vascular density (E), vascular coverage (F), the fraction of vessels that immunostain for Claudin5 (G), and the fraction of vessels that immunostain for PLVAP (H), for the genotypes shown in (A–D). Scale bars for columns 1 and 4, 400 μm. Scale bars for columns 2, 3, and 5, 200 μm.

Figure 5.

Figure 5—figure supplement 1. Loss of Dlg1 in endothelial cells in P7 retinas: marker expression and biotin leakage.

Figure 5—figure supplement 1.

(A,B) P7 retinas, with the boxed region in column 1 shown at higher magnification in columns 2 and 3, and the boxed region in column 4 shown at higher magnification in column 5. Mice were injected IP with 2–3 mg of sulfo-NHS-biotin 1–2 hr before sacrifice. Scale bars for columns 1 and 4, 400 μm. Scale bars for columns 2, 3, and 5, 200 μm.