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. Author manuscript; available in PMC: 2019 May 8.
Published in final edited form as: Biochem J. 2019 Jan 15;476(1):115–135. doi: 10.1042/BCJ20180577

Figure 1.

Figure 1.

In vitro crosslinking reactions of apo and Ag(I)- CusS(39–387) wild-type and mutants. Apo- and Ag(I)- CusS(39–387) samples (10 μM) in the presence and absence of BS3 crosslinker were analyzed by Western analysis. (A) The Western blot analysis was performed using anti-Strep tag antibody. The expected monomer (~18 kDa) CusS(39–387) is indicated by (*) and the expected dimer (~36 kDa) is indicated by (**). Experiment was repeated twice; a representative experiment is shown. (B) The relative densities of the dimers (**) were calculated and normalized to the apo proteins. Student’s t test was used to calculate the P value. Error bars indicate standard deviations of duplicate experiments.