Figure S1.

Promoters with SuRE Activity Inactive in Endogenous Context, Related to Figure 1

(A) Percentage of promoters active in episomal plasmid context (log₁₀(SuRE) > 0) with no measurable activity in native context (log₁₀(GRO-cap) < −3), for LAD promoters and a subset of iLAD promoters matched on SuRE activity.

(B) Distributions of expression levels in episomal plasmid context (log₁₀(SuRE)) of matched promoter sets used in (A).

(C) Comparison between endogenous (log₁₀(CAGE)) and episomal (log₁₀(SuRE)) expression for all promoters in LADs (red) and iLAD regions (blue). Similar to Figure 1B, but with CAGE instead of GRO-cap. Blue and red lines are sliding window averages with a bin size of 501 promoters along the SuRE expression axis.

(D) Same analysis as in Figures 1B and S1C, but with log₁₀(PRO-seq) as a measure of endogenous expression.

(E) Comparison between CAGE and SuRE for the three classes of LAD promoters as defined in Figure 1C.

(F) Comparison between PRO-seq and SuRE for the three classes of LAD promoters as defined in Figure 1C.