Figure S1.

Insulin Affects Circadian Gene Expression In Vivo, Related to Figure 2

(A) Bioluminescent recordings of PER2::LUC mice following i.p. injection of insulin (2.25 IU/kg) or glucose (3 g/kg) or insulin and glucose in combination. Arrow indicates timing of i.p. injection. Grey line indicates timing of PER2::LUC peak in the vehicle-treated group (n ≥ 4, representative). Glucose/insulin and vehicle traces repeated from Figure 2H. (B) Quantification of the change in PER2::LUC signal at 1 h following i.p. injection (n ≥ 4, 1-way ANOVA, Tukey’s multiple comparisons test). (C) Circulating blood glucose sampled from mouse-tail following i.p. injection as in Figure S1A (n = 4, mean ± SEM, 2-way ANOVA, Dunnett’s multiple comparisons test versus t = 0 reported). (D) Quantification of difference in phase of PER2::LUC expression in vivo between the vehicle and insulin/glucose injected groups, from Figure S1A (Welch’s t test, one tailed). Values are relative to the vehicle-treated group.