Figure 1.
Ablation Triggers Restorative Cell Divisions in Inner Adjacent Cells
(A) Cell types in the Arabidopsis root meristem. Inset shows magnification of stem cell niche.
(B) 3D reconstruction of a single ablated endodermal cell in SCR::SCR-YFP root just after ablation. Propidium iodide (PI) stains cell walls and is not permeant to living cells and therefore was used to identify the ablated cell and intact neighboring cells. Shortly after ablation, PI becomes oversaturated and thus can partly overlap also with neighboring non-ablated cells. Later, PI staining is more restricted to the collapsed eliminated cell (see Figures S1C and S1D).
(C) Anticlinal division of cells in root meristem (gray arrow).
(D–G) Periclinal divisions of the inner adjacent cells (green arrows) after ablation. Ablation in LRC (D), epidermis (E), cortex (F), and endodermis (G) is shown. Total number of ablations: n = 30–60 per cell type.
LRC, lateral root cap; Ep, epidermis; Co, cortex; En, endodermis; P, pericycle. Red asterisks: sites of ablation.