Figure 4.
Restorative Cell Divisions Generate Daughter Cells with Distinct Cell Fates
(A) Expression domains of marker lines in Arabidopsis root meristem.
(B) Ablation of LRC cells. Expression of LRC marker SMB::SMB-GFP is restored in newly formed LRC cells. Total number of ablations: n = 5 for all time points.
(C and D) Ablation of epidermal cells. Expression of cortex and endodermis marker J0571 is downregulated in the outer daughter cells of periclinally divided cortex cells (C), while epidermis marker WER::GFP is upregulated (D). Numbers of ablations per time point: n = 19–77 (J0571), n = 16–66 (WER::GFP).
(E) Ablation of cortex. Expression of cortex marker Co2::HYFP is upregulated in outer daughter cells of periclinally divided endodermis cells, while endodermis marker SCR::SCR-YFP is downregulated (see Figure S4A). Co2::HYFP; n = 43–79 ablations per time point.
(F) Ablation of endodermis. Expression of SCR::SCR-YFP is upregulated in outer cells of periclinally divided pericycle cells. n = 13–23 ablations per time point.
(G–J) Quantification of fluorescent signals of marker gene expressions in periclinally divided cells after ablation. Relative signal intensity between outer/inner daughter cells in periclinally divided epidermis (G) and average fluorescent signal in cortex (H), endodermis (I), and pericycle (J) at different time points. Outer daughters were measured if the inner adjacent cells divided multiple times (as seen in D). Data are represented as mean ± SEM.
Red asterisks: sites of ablation. Roots were stained with PI.