Figure 5.
Re-activation of SHR/SCR-CYCD6;1 Module during Restoration of Cortex
(A) SHR/SCR-CYCD6;1 module in ground tissue development (Fisher and Sozzani, 2016, Sozzani et al., 2010).
(B and C) Ablation of epidermal cells (B, left) and ground tissue (B, right) in shr-1 mutant. Quantification of periclinal divisions in scr-3 and shr-1 (C). Data are represented as weighted mean (bar) and individual experiments (dots, area indicates sample size); p values from CLR for scr-3: 0.00234; shr-1: 8.61E−9.
M, ground tissue layer formed in shr mutants.
(D and E) Expression of CYCD6;1::GFP is upregulated in periclinally divided endodermis cell after ablation of cortex (for the translational fusion, see Figure S5A) (D), while SHR::SHR-GFP is downregulated before periclinal division (see also Figure S5B) (E). Total number of ablations: n = 109 (CYCD6;1), n = 101 (SHR).
Red asterisks: sites of ablation. Roots were stained with PI.