Figure S4.

Cell Fates of Inner and Outer Adjacent Cells of Ablation, Related to Figure 4

(A) Ablation of a cortex cell results in decreased expression of endodermis marker SCR::SCR-YFP. Total number of ablations; n = 42 (8 h), n = 47 (12 h), n = 95 (16 h), n = 63 (24 h), n = 47 (32 h) - see increased cortex marker Co2::HYFP expression in Figure 4E.

(B and C) Ablation of endodermal cell results in upregulation of SCR::SCR-YFP in pericycle (B). Ablation of cortex and endodermis results in nuclear localization of SHR::SHR-GFP (C) in outer daughter cells of periclinally divided pericycle cells. Total number of ablations; n = 71 (16 h), n = 55 (24 h), n = 22 (32 h), n = 22 (38 h).

(D-I) Rarely induced restorative divisions in outer adjacent cells did not trigger cell fate change in any of the daughter cells. After 16 h and 38 h of cortex ablation, expression pattern of WER::GFP in outer adjacent periclinally divided daughter cells did not change (D and E). Expression patterns of LRC marker E4722 (F, 16 h; n = 52/52, 38 h; n = 43/45), SCR::SCR-YFP (G, 16 h; n = 24/24, 38 h; n = 37/37), and WER::GFP (H and I) after 16 h and 38 h of ablation did not change in the presence of 1 μM NAA, which increased occurrence of restorative divisions at the outer adjacent side. Relative fluorescence signal was calculated as follows; WER::GFP signals in the periclinally divided cells were divided by non-periclinally divided epidermal cells. Sample numbers are as follows; in D and E (16 h; n = 14, 38h; n = 9), in H and I (16 h; n = 10, 38h; n = 7). The error bars show SD.

Red asterisks: sites of ablation. Roots were stained with propidium iodide.