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. 2019 Mar 3;33(2):313–323. doi: 10.21873/invivo.11477

Figure 2. Stimulating effect of n-butyl acrylate (nBA), n-butyl methacrylate (nBMA) and ethyleneglycol dimethacrylate (EGDMA) on cyclooxygenase- 2 (Cox2), nitric oxide synthase 2 (Nos2) and heme oxygenase 1 (Ho1) gene expression in RAW264.7 cells. The cells were incubated for 3.5 h with each compound at a concentration of 0.1-1 mM, and then their total RNAs were prepared. Each cDNA was synthesized, and the expression level of each mRNA for Cox2, Nos2 and Ho-1 was determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE <15%. *Significantly different at p<0.01 vs. control group.

Figure 2