Figure 3. Stimulating effect of 2-hydroxyethyl methacrylate (2-HEMA), methyl methacrylate (MMA) and triethyleneglycol dimetacrylate (TEGDMA) on cyclo-oxygenase-2 (Cox2), nitric oxide synthase 2 (Nos2) and heme oxygenase 1 (Ho1) gene expression in RAW264.7 cells. The cells were incubated for 3.5 h with each compound at a concentration of 1-100 mM, and then their total RNAs were prepared. Each cDNA was synthesized, and the expression level of each mRNA for Cox2, Nos2 and Ho-1 was determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE <15%. *p<0.05 vs. control group.