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. Author manuscript; available in PMC: 2019 May 9.
Published in final edited form as: ACS Nano. 2018 Jun 4;12(7):6563–6576. doi: 10.1021/acsnano.8b01308

Figure 10. Murine αDHFR2 Immune Response.

Figure 10.

Freshly isolated spleen cells from naïve mice were incubated with DHFR2 protein, non-targeted CSANs and CpG 1826 (positive control) for 24 hours and monitored for (A) CD40 and CD86 expression as well as (B) IL-6 release. Mice were injected with non-targeted CSANs and (C) monitored on day 13, 21 and 30 since the first immunization. Sera were collected for estimation of αDHFR IgG antibodies by ELISA. Data were shown as absorbance values at 450 nm wavelength at appropriate dilutions. Each bar represents the mean value of absorbance value OD450 ± SD for each group (n=4). Values of p < 0.05 were considered significant and were indicated as follows: *p < 0.05. Statistical differences between the groups were determined by one-way ANOVA analysis and Student t test.