A. IHC on serial liver sections shows same tumor foci to be positive for GS and p-mTOR-S2448 in the Met-β-catenin, Kras-β-catenin and Nrf2-β-catenin models (50×). Cholangiocarcinoma (CCA) occurring in Kras-β-catenin model are negative. IHC on serial sections also shows hepatoblastoma (HB) to be positive for GS and p-mTOR-S2448 in Yap-β-catenin model (50×).
B. IHC on serial liver sections shows same tumor foci to be negative for GS and p-mTOR-S2448 in the Met-WT-β-catenin HCC model generated by co-expressing wild-type non-mutant β-catenin and c-Met (50×).