Table 2.

PCR reaction conditions.

Herculase Reaction
Water	37.5 μL
Buffer	5 μL
DNA	1.5 μL (~150 ng)
Primer Forward	1 μL (10 μM stock)
Primer Reverse	1 μL (10 μM stock)
dNTPS	2 μL (10 mM stock)
Polymerase	2 μL

Herculase Thermocycler Conditions
Temperature	Time	Repeats
92°C	2 min	1
92°C	30 s	30
55°C	30 s
68°C	10 min
68°C	15 min	1
4°C	Indefinite	Indefinite

Herculase II Reaction
Water	30.5 μL
Buffer	10 μL
DNA	1.5 μL (~150 ng)
Primer Forward	2.5 μL (10 μM stock)
Primer Reverse	2.5 μL (10 μM stock)
dNTPS	2 μL (10 mM stock)
Polymerase	1 μL

Herculase II Thermocycler Conditions
Temperature	Time	Repeats
92°C	2 min	1
92°C	30 s	10
55°C	30 s
68°C	11 min
92°C	30 s	20
55°C	30 s
68°C	11 min + 20 s/cycle
68°C	12 min	1
4°C	Indefinite	Indefinite